An evaluation of the heat precipitation method for plasma fibrinogen estimation.
نویسندگان
چکیده
A rapid heat precipitation micromethod for the estimation of plasma fibrinogen is described. Its validity and reproducibility have been extensively tested. The method correlates well with a more generally used thrombin-clotting technique over a wide range of fibrinogen levels.
منابع مشابه
An Evaluation of Technics for the Separation and Estimation of Plasma Fibrinogen.
A proposed heat precipitation and thrombin interaction technic for fibrinogen isolation has been compared with a sulfite fractionation technic. All three methods exhibit a high degree of correlation. Fibrinogen, which has been separated from plasma by these technics, may be quantitated by the colorimetric modified biuret and phenol-biuret reaction. For direct measurement of fibrinogen, heat pre...
متن کاملRe-evaluation of heat precipitation method for plasma fibrinogen estimation: effect of abnormal proteins and plasma viscosity.
The heat precipitation method (Aberdeen method) was compared with the Ratnoff and Menzie method of fibrinogen assay in 320 donors, including normals and patients suffering from malignant melanoma, renal failure, hypertension, multiple myeloma, etc. Excellent correlation (r=0-8287, p less than 0-000 000 1) was found between these two methods. However, on some occasions individual low results wer...
متن کاملAfibrinogenemia and a circulating antibody against fibrinogen in a Bichon Frise dog.
A 1.5-year-old female Bichon Frise dog was evaluated for a life-threatening hemorrhagic condition that occurred after ovariohysterectomy, requiring 4 whole-blood transfusions. A hemostatic profile, including activated clotting time (ACT), one-stage prothrombin time (OSPT), activated partial thromboplastin time (APTT), buccal mucosal bleeding time, and specific assays (heat-precipitation microhe...
متن کاملAn evaluation of turbidimetric technics for estimation of plasma fibrinogen.
Studies on precipitation and turbidimetric methods for fibrinogen determination employing Na2SO3, Na2SO4, (NH4) 2S04 and a mixture of phosphate salts are presented. Optimal reaction conditions for these technics have been verified. The amounts of plasma fibrinogen precipitated by the sulfite and phosphate methods are in closer agreement than either are with values obtained by the (NH4)2S04 and ...
متن کاملClinical nephelometry. 1. New, accurate, heat-precipitation nephelometric method for rapid determination of plasma fibrinogen.
We have developed an accurate procedure for measurement of plasma fibrinogen. Turbidity resulting from heating (56#{176}C, 20 mm) plasma diluted with a new “zwitterionic” buffered-saline reagent (pH 5.6) is nephelometrically measured (600 nm). We evaluated the method both by confirming the accuracy of its prestandardization and calibration on a new clinical nephelometer, and by comparative stan...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of clinical pathology
دوره 24 9 شماره
صفحات -
تاریخ انتشار 1971